Table 1. Primer sequence and reaction condition.

SNPs	Primer sequence	Annealing temperature	Restriction enzyme	Product size (bp)
rs2228570	F: 5′-AGCTGGCCCTGGCACTGACTCTGGCTCT-3′	60.0°C	BseG I	CC: 267 bp
	R: 5′-ATGGAAACACCTTGCTTCTTCTCCCTC-3′			CT: 267+204+63 bp
				TT: 204+63 bp
rs3782905	F: 5′-AAGACATGGTGTCTGCTTCA-3′	56.0°C	HpyF3 I	CC: 223+81 bp
	R: 5′-GGTTAGATCGATATGTTTGA-3′			CG: 304+223+81 bp
				GG: 304 bp
rs7041	F: 5′-AAATAATGAGCAAATGAAAGAAGAC-3′	56.0°C	BsuR I	TT: 482 bp
	R: 5′-CAATAACAGCAAAGAAATGAGTAGA-3′			TG: 482+298+184 bp
				GG: 298+184 bp
rs11568820	F1: 5′-AGGATAGAGAAAATAATAGAAAACATT-3′	45.0°C		GG: 297+110 bp
	R1: 5′-AACCCATAATAAGAAATAAGTTTTTAC-3′			GA: 297+235+110 bp
	F2: 5′-TCCTGAGTAAACTAGGTCACAA-3′			AA: 297+235 bp
	R2: 5′-ACGTTAAGTTCAGAAAGATTAATTC-3′