Skip to main content
. 2014 Dec 26;9(12):e116165. doi: 10.1371/journal.pone.0116165

Figure 1. NO stabilizes and increases SIRT1 protein expression.

Figure 1

(A) HUVECs were transfected with GFP or eNOS adenovirus for 48 h; (B) HUVECs were treated with A23187 (1 µM) for the indicated time; (C) HUVECs were treated with NONOate (50 µM) for the indicated time; (D) HUVECs were treated with NONOate (50 µM) for the indicated time; SIRT1 mRNA levels were determined by RT-PCR; (E) HUVECs were treated with CHX (5 µM) for the indicated time, followed by incubation of NONOate (50 µM) for 4 h; (F) HUVECs were treated with the indicated concentrations of MG132 for 6 h. The western blots are representative of three independent experiments. *represents p<0.05 vs control (n = 3); NS, not significant. GFP, green fluorescent protein; eNOS, endothelial nitric oxide synthase; Ad, Adenovirus; CHX, cycloheximide.