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. 2014 Dec 26;9(12):e116165. doi: 10.1371/journal.pone.0116165

Figure 3. ULK1 mediates the NO-induced SIRT1 upregulation.

Figure 3

(A) MEF from the Ulk1 −/−, Ulk2 −/−, Ulk1/2 −/−, and WT mice were transfected with GFP or eNOS Adenovirus for 48 h; (B) MEF from the Ulk1 −/−, Ulk2 −/−, Ulk1/2 −/−, and WT mice were treated with NONOate (50 µM) or A23187 (1 µM) for 4 h; (C) HUVECs were transfected with control or ULK1 SiRNA for 48 h, then treated with A23187 (1 µM) for 4 h; (D) HUVECs were transfected with control or ULK1 SiRNA for 48 h, then treated with NONOate (50 µM) for 4 h. The western blots shown are representative of three independent experiments. *represents p<0.05 vs control (n = 3); #represents p<0.05 vs A23187 or NONOate alone (n = 3); NS, not significant. GFP, green fluorescent protein; eNOS, endothelial nitric oxide synthase; Ad, Adenovirus; Si-SiRNA.