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. 2014 Dec 26;9(12):e116165. doi: 10.1371/journal.pone.0116165

Figure 5. ULK1 regulates SIRT1 protein expression via 26S proteasomes.

Figure 5

(A) HUVECs were transfected with control or ULK1 SiRNA for 48 h, then treated with epoxomicin (0.1 µM) for 4 h; (B) HUVECs were transfected with control or ULK1 siRNA for 48 h, SIRT1 mRNA levels were determined by RT-PCR; (C) HUVECs were transfected with control or ULK1 or β-TrCP1 siRNA for 48 h. The western blots are representative of three independent experiments. *represents p<0.05 vs control (n = 3); # represents p<0.05 vs si-ULK1 alone (n = 3). Si, siRNA.