Figure 1. p62 regulates HP trafficking in a non-cell autonomous manner.

(A) CFU-C content in the PB (PB) of Wt or p62^−/− mice (n=8–10 mice per group). Values represent average of three independent experiments. (B) Experimental set up. PB mononuclear cells (PBNC) from CD45.2⁺ Wt or p62^−/− mice were mixed with CD45.1⁺ B6.SJL^{Ptprca Pep3b/BoyJ}Wt bone marrow (BM) cells and competitively transplanted into lethally irradiated CD45.1⁺ B6.SJL^{Ptprca Pep3b/BoyJ} mice. (C) CD45.2⁺ chimera in the PB of recipient mice (5–8 mice per group) after 6 weeks of competitive transplantation. (D) Frequency of hematopoietic progenitors in spleen of Wt or p62^−/− mice (n=3 mice per group). (E) Experimental set up. BM cells from CD45.1⁺ B6.SJL^{Ptprca Pep3b/BoyJ} mice were non-competitively transplanted into lethally irradiated CD45.2⁺ Wt or p62^−/− mice to generate chimeric Wt HM or HM p62^−/− mice.(F) Absolute numbers of CFU-C present in the PB of Wt HM or HM p62^−/− mice (n=4–7 mice per group). (G) Experimental set up.BM cells from CD45.1⁺ B6.SJL^{Ptprca Pep3b/BoyJ} mice were non-competitively transplanted into lethally irradiated CD45.2⁺ Wt or p62^−/− mice to generate chimeric Wt HM or HM p62^−/− mice. BM cells isolated from primary Wt HM or HM p62^−/− mice were transplanted into lethally irradiated CD45.2⁺ to generate secondary Wt recipients. (H) Absolute numbers of CFU-C present in the PB of secondary Wt recipient mice (n=4–6 mice per group). Values represent mean ± SEM. p=ns. (I–J) Homing (%) of Wt ST-HSC (I), LSK (J) and LK (K) BM cells to non-myeloablated BM from Wt or p62^−/− mice. A minimum of 5 mice were analyzed per group. Values represent mean ± SEM. * p<0.05.