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. Author manuscript; available in PMC: 2015 Dec 24.
Published in final edited form as: Cell Rep. 2014 Dec 11;9(6):2071–2083. doi: 10.1016/j.celrep.2014.11.021

Figure 1. Endocardial deletion of Hand2 results in a VSD, hypotrabeculation, hypoplastic RV, IVS defects and TA.

Figure 1

Hand2 ISH of RV section from E10.5 control, Tie2-Cre H2CKO, and Nfatc1CreH2CKO, respectively (A–C). Wholemount view of E12.5 control heart, Tie2-Cre H2CKO, and Nfatc1Cre H2CKO, respectively (D–F). R26RlacZ stained sections from E12.5 Tie2-Cre(+) control embryo (G), Tie2-Cre H2CKO with TA (H). R26RlacZ stained E12.5 Tie2-Cre(+) control embryo (I), and Tie2-Cre H2CKO with DILV (J). R26RlacZ stained sections from E12.5 Nfatc1Cre control embryo (M), and Nfatc1Cre H2CKO (N; arrows denote multiple IVSs). Alcian blue staining of E12.5 Tie2-Cre(+) control AV cushions (K), and Tie2-Cre H2CKO (L). Asterisk denotes abnormalities in AV cushion shape and position. SAVC, superior atrioventricular cushion; IAVC, inferior atrioventricular cushion; tv, tricuspid valve; mv, mitral valve. Scale bars in A – C represent 100μm; scale bars in G – N represent 250μm.