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. Author manuscript; available in PMC: 2015 Dec 24.
Published in final edited form as: Cell Rep. 2014 Dec 11;9(6):2071–2083. doi: 10.1016/j.celrep.2014.11.021

Figure 3. Hand2 functions downstream of Notch1 and the direct Notch1 target EphrinB2 during trabeculation.

Figure 3

Wholemount Hand2 ISH in wild type (A) and RBPJk knockout embryos (B). Arrows indicate endocardial Hand2 expression in control (A) and loss of expression in RBPJk knockouts (B). Hand2 ISH (C) shows robust endocardial Hand2 expression that is downregulated within RBPJk−/− endocardium (D). Hand2 ISH (E) revealed a similar downregulation of Hand2 within the endocardium of Tie2-Cre EfnB2CKO hearts (F Arrows denote endocardium). PM, pharyngeal mesenchyme; PEO, proepicardial organ. qPCR on RNA isolated from ventricles confirms significant (* p≤0.05) downregulation of Hand2 and Nrg1 in Tie2-Cre EfnB2CKO hearts (G). Scale bars in C and D represent 50μm; scale bars in E and F represent 100μm.