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. Author manuscript; available in PMC: 2015 Dec 24.
Published in final edited form as: Cell Rep. 2014 Dec 11;9(6):2071–2083. doi: 10.1016/j.celrep.2014.11.021

Figure 4. Hand2 directly regulates Nrg1.

Figure 4

Sequence alignments reveal three Hand2 consensus sites that are highly conserved among mammals (A). ChIP of the NRG1 promoter (B). In-vitro transcribed and translated Hand2 and E12 used in EMSAs (C). EMSAs for D-box 1 (D) and E-box 3 (E) show robust and specific binding of Hand2-E12 heterodimers. No binding is observed for site 2. Luciferase reporter assays with a deletion series of the Nrg1 promoter. Red numbers denote positions of the Hand2 consensus sites; green arrows denote positions of the transcription start sites; black arrows, location of ChIP primers (F). Transactivation of 500bp Nrg1 promoter reporter (G; *p≤0.05).