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. Author manuscript; available in PMC: 2015 Dec 24.
Published in final edited form as: Cell Rep. 2014 Dec 11;9(6):2071–2083. doi: 10.1016/j.celrep.2014.11.021

Figure 5. Conditional CAG-CAT-Hand2 transgene expression in Nfatc1Cre EfnB2 CKOs partially improves trabeculation and Nrg1 expression.

Figure 5

Wholemount images of E10.5 control, CC-H2(+) control, EfnB2 CKO, and CC-H2(+); EfnB2 CKO embryos (A–D). Hand2 section ISH (E–H). Arrow in G indicates loss of Hand2; arrow in H indicates restoration of endocardial Hand2 via CC-H2. Nrg1 section ISH (I–L). Arrow in K indicates loss of Nrg1 in EfnB2 CKOs; arrow in L indicates restored Nrg1 expression in EfnB2 CKOs that are also CC-H2(+).Bmp10 section ISH (M–P). Arrow in O indicates Bmp10 expressing myocardium and lack of trabeculation; arrow in P indicates restoration of Bmp10 expressing trabeculations. qPCR analysis of isolated E10.5 ventricles (Q). Asterisks denote significant difference (p≤0.05). CC-H2, CAG-CAT-Hand2. Scale bars in E – P represent 100μm.