Skip to main content
. Author manuscript; available in PMC: 2015 Dec 24.
Published in final edited form as: Cell Rep. 2014 Dec 11;9(6):2071–2083. doi: 10.1016/j.celrep.2014.11.021

Figure 7. Hand2 regulates Vegf signaling.

Figure 7

Differentially expressed genes that were analyzed for direct regulation by Hand2 (A). ChIP of a region within the 500bp VegfR3 promoter (B). EMSA demonstrates Hand2-E12 heterodimers specifically bind an oligo corresponding to an E-box within the ChIP amplicon of the VegfR3 promoter (C). Black arrow indicates E12 homodimer binding, red arrow indicates Hand2-E12 heterodimer binding, and asterisk indicates nonspecific binding. Hand2-E12 heterodimers repress significantly (p≤0.05) a luciferase reporter containing the 500bp VegfR3 promoter (D). Hand2-3xFLAG ChIP-seq demonstrates a prominent region of enrichment approximately 356kb upstream of the Nrp1 coding region (E). A luciferase reporter containing this potential enhancer region is significantly (p≤0.05) trans-activated 5-fold by H2-E12 heterodimers (F). ISH demonstrates strong myocardial expression of VegfA within the compact zone of control E13.5 hearts (G). Red dots denote the border between compact and trabecular myocardium. In H2CKOs, the distinction between VegfA expression levels in compact versus trabecular myocardium is more poorly defined (H). Scale bars in G and H represent 100μm.