FIG 2.

Coomassie blue-stained SDS-PAGE analyses of TCs pulled down by IP using anti-BoNT/A1 or anti-BoNT/A2 capture antibodies with C. botulinum subtypes A1 (A), A2 (B), A3 (C), and A5 (D). Abbreviations: NR, nonreduced samples; R, samples reduced with 100 mM DTT. (A and D) WT, 150-kDa holotoxin; AB, full-length Ab; ABLC, light chain of Ab; 1, NTNH; 2, truncated NTNH; 3, BoNT Hc; 4, heavy chain of Ab; 5, BoNT/A Lc; negative controls, beads with anti-BoNT/A1 Ab only without crude culture extract and beads with crude culture extract only without Ab. (B and C) WT, 150-kDa holotoxin; AB, full-length Ab; ABLC, light chain of Ab; 1, NTNH; 2, BoNT Hc; 3, heavy chain of Ab; 4, BoNT Lc; negative controls, beads with anti-BoNT/A2 Ab without crude culture extract and beads with crude culture extract without Ab.