FIG 5.

(A) PCoA (weighted UniFrac) shows a clear separation of toilet seat-associated microbial communities based on gender. (B) Top 10 most abundant OTUs that show significant differences in abundance between the toilet seats in male and female restrooms (most resolved taxonomic annotations are shown on the x axis). (C) Toilet seat samples cluster separately based on restroom usage frequency (high versus low). (D) Seven OTUs that exhibit significantly different abundances between high- and low-usage frequencies (most resolved taxonomic annotations are listed along the x axis).