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Effect of pH on residual activity of AtzF and its variants. Differences in the residual activity of AtzF, AtzF467, and AtzF H488A at pH 7 (A) and pH 9 (B) were compared. Enzymes were incubated in pH 7 or pH 9 buffers for 5 min at temperatures ranging from 30 to 70°C before testing for residual activity.
