FIG 1.
2D 1H/1H COSY NMR spectrum (A) and 2D 1H/13C HSQC NMR spectrum (B) of EPS produced by M. leachii strain PG50T. The H-1 signal at 4.873 ppm (1,2J = 7.83 Hz) and that of C-1 at 103.26 ppm were typical of d-glucose residues with β-pyranoside configuration (dark arrow). The single signal for C-2 (83.91 ppm) present in the 13C spectrum (light arrow) was indicative of a linear polymer. This spectrum is typical of a linear β(1→2)-glucopyranose polymer. Identical spectra were obtained with M. capricolum subsp. capripneumoniae EPS.