TABLE 1.
Representative Brucella strains for which the GAD phenotype (Rice test) was assayed and exported GABA was measureda
| Species and strain | Natural host | Protein length (aa) |
GAD Rice testb | Exported GABA (mM) | |
|---|---|---|---|---|---|
| GadB | GadC | ||||
| B. abortus | |||||
| ATCC 23448 bv. 1 (544) | Cattle | 155/286 | 510 | − | 0.00 ± 0.00 |
| ATCC 27565 bv. 1 (S19) | Cattle | 155/274 | 510 | − | 0.00 ± 0.00 |
| B. canis | |||||
| ATCC 23365 bv.1 | Dog | 319/136 | 426 | − | 0.00 ± 0.00 |
| B. ceti | |||||
| B1/94 bv.1 | Porpoise | 464 | 513 | + | 0.25 ± 0.00 |
| M490/95/1 | Common seal | 464 | 513 | + | 0.53 ± 0.01 |
| M644/93/1 | Common dolphin | 464 | 513 | + | 1.83 ± 0.37 |
| B202R | Minke whale | ND | ND | + | 0.26 ± 0.08 |
| B. inopinata | |||||
| BO1 | Human | 464 | 509 | + | 0.12 ± 0.04 |
| B. inopinata-like | |||||
| BO2 | Human | 464 | 471c | + | 0.02 ± 0.02 |
| B. melitensis | |||||
| ATCC 23456 bv. 1 (16 M) | Goat | 167/304 | 510 | − | 0.00 ± 0.00 |
| B. microti | |||||
| CCM4915 | Common vole | 464 | 510 | + | 0.20 ± 0.04 |
| CCM4916 | Common vole | ND | ND | + | 0.65 ± 0.09 |
| B. neotomae | |||||
| 5K33 | Desert woodrat | 263/201 | 510 | − | 0.00 ± 0.00 |
| B. ovis | |||||
| ATCC 25840 bv. 1 (63/290) | Sheep | 455 | 291/206 | +w | 0.00 ± 0.00 |
| B. pinnipedialis | |||||
| M163/99/10 | Hooded seal | 464 | 513 | + | 0.33 ± 0.06 |
| B2/94 bv. 1 | Common seal | 464 | 510 | +w | 0.04 ± 0.04 |
| Brucella spp. | |||||
| Br1 | African bullfrog | ND | ND | +s | 0.17 ± 0.04 |
| Br2 | African bullfrog | ND | ND | + | 1.18 ± 0.47 |
| Br3 | African bullfrog | ND | ND | + | 0.25 ± 0.18 |
| Br4 | African bullfrog | ND | ND | + | 1.04 ± 0.25 |
| B. suis | |||||
| ATCC 23444 bv. 1 (1330) | Swine | 319/136 | 426 | − | 0.00 ± 0.00 |
| ATCC 23445 bv. 2 (Thomsen) | Swine | 454 | 336d | + | 0.00 ± 0.00 |
The natural host of each strain and the lengths of their putative GadB and GadC proteins are provided. For GadB, the numbers before and after the character “/” indicate the respective sizes of the two consecutive fragments annotated as decarboxylases in the presence of a stop codon. ND, not determined (sequence unavailable).
The qualitative GAD test is based on the color change of the Rice reagent. Following the increase in pH that accompanies the decarboxylation of glutamic acid, the pH indicator bromocresol green turns from yellow (negative) to blue (positive). −, negative (yellow); +, positive (blue); +w, weakly positive (green); +s, slowly positive (blue color develops slowly during incubation of >8 h).
Shorter form, truncated at N terminus, with probably decreased ability to export GABA.
Shorter form, truncated at N terminus, probably unable to export GABA.