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The quenching constants of NR-DNA by IBT at different temperatures ranging from 0 to 4.9×10-5 M, [DNA] = 9×10-5 M, [NR] = 3×10-5 M, Binding constants (Kf), number of binding sites (n) and relative thermodynamic parameters of IBT-DNA system.
| Temperature (K) | Ksv(L/mol)×104 | Kq(L/mol)×1012 | Kf | n | ΔG (kJ/mol) | ΔH (kJ/mol) |
ΔS (J/molK) |
|---|---|---|---|---|---|---|---|
| 310 | 1.14 | 1.14 | 2.82×104 | 1.10 | -73.167 | ||
| 290 | 2.57 | 2.57 | 1.04×105 | 1.13 | -71.664 | -49.87 | -75.152 |
| 298 | 2.04 | 2.04 | 7.52×104 | 1.13 | -72.265 | ||
| 303 | 1.34 | 1.34 | 4.77×104 | 1.12 | -72.641 |
