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. 2015 Winter;14(1):177–187.

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© 2015 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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PCR analysis of genomic DNA from zeocin/blasticidin-resistant Sp2.0 cells. PCR products were electrophoresed on a 1% agarose gel. Lane 1, 1kb DNA markers; lane 2, amplification of heavy chain gene from DNA of resistant Sp2.0 cells; lane 3, amplification of variable region of heavy chain gene from DNA of resistant Sp2.0 cells; lane 4, amplification of light chain gene from DNA of resistant Sp2.0 cells; lane 5, amplification of variable region of light chain gene from DNA of resistant Sp2.0 cells; lane 6, amplification of variable region of heavy chain from recombinant vector pFUSE-CHIg-hG4-VH; lane 7, amplification of variable region of light chain from recombinant vector pFUSE-CLIg-hk-VL.