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. 2015 Winter;14(1):177–187.

Figure 3.

Figure 3

RT-PCR analysis of total RNA isolated from untransfected and rSp2.0. PCR products were electrophoresed on a 1% agarose gel. Lane 1, 1kb DNA markers; lane 2, amplification of variable region of heavy chain from recombinant vector pFUSE-CHIg-hG4-VH; lane 3, amplification of variable region of heavy chain gene from RNA of rSp2.0; lane 4, amplification of variable region of light chain gene from RNA of rSp2.0; lane 5, amplification of variable region of heavy chain gene from RNA of untransfected Sp2.0; lane 6, amplification of variable region of light chain gene from RNA of untransfected Sp2.0.