Figure 4.

SDS–PAGE and Western blot analysis of expressed IgG₄. The concentrated culture supernatant was separated on 8% resolving gel under non-reducing condition. Purified protein was analyzed under reducing condition on 12% resolving gel. The gels were stained with Coomasie Blue. In Western blot analysis, the blots were probed with a HRP-conjugated chicken Anti-Human IgG. Western blots were visualized by ECL. (A) Coomassie stained gel under non-reduced condition. Lane 1, supernatant of rSp2.0 cells; lane 2, supernatant of untransfected Sp2.0 cells; lane 3, positive control; lane 4, marker (B) Western blot analysis under non-reduced condition. Lane 1, marker; lane 2, supernatant of untransfected Sp2.0 cells (30 µg/mL); lane 3, supernatant of untransfected Sp2.0 cells (10 µg/mL); lane 4, supernatant of rSp2.0 cells (30 µg/mL); lane 5, supernatant of rSp2.0 cells (10 µg/mL); lane 6, positive control (C) Coomassie stained gel under reduced condition. Lane 1, marker; lane 2, purified IgG₄, lane 3, positive control (D) Western blot analysis under reduced condition. Lane 1, marker; lane 2, purified IgG₄, lane 3, positive control.