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. 2004 Jun;70(6):3213–3221. doi: 10.1128/AEM.70.6.3213-3221.2004

FIG. 5.

FIG. 5.

(A) Overexpression of esterase from L. casei CL96 in E. coli BL21(DE3)/pLysS. Protein samples were separated in an SDS-12% polyacrylamide gel and stained with Coomassie brilliant blue R-250. Lanes: M, molecular mass standards; 1, E. coli BL21(DE3)/pLysS bearing vector pET29a; 2 to 7, E. coli BL21(DE3)/pLysS containing pET29a-estI after 2, 4, 6, 8, 10, and 12 h of induction, respectively; P, purified esterase (3 μg). (B) Staining of a 12% nondenaturing gel for activity of esterase from L. casei CL96 in E. coli BL21(DE3)/pLysS. Lanes 1 to 4, soluble fractions of crude lysates from 10, 8, 6, and 4 h after induction, respectively.