TABLE 1.
Strain or plasmid | Relevant characteristic(s)a | Source or reference |
---|---|---|
Strains | ||
E. coli Top10F′ | F′ [lacIq Tn10 (Tetr)] mcrA Δ(mrr-sdRMS-mcrBC) φ80 lacZ; cloning host | Invitrogen Canada |
E. coli BL21(DE3)/pLysS | F−ompT hsdSB(rB− mB−) dcm (DE3); cloning and expression host for estI gene | Invitrogen Canada |
L. casei CL96 | Wild type; source of estI gene | This study |
M. extorquens ATCC 55366 | Wild type; cloning host for expression of estI gene | American Type Culture Collection |
P. pastoris X-33 | Wild type; cloning host for expression of estI gene | Invitrogen Canada |
Plasmids | ||
pCWL70 | Positive selection vector; Ampr Emr | Choi and Lee, Abstr. 101st Gen. Meet. Am. Soc. Microbiol. 2001 |
pCESTa | 3.3-kb BamHI fragment cloned into BamHI site of pCWL70; Ampr | This study |
pET29a | E. coli expression vector; Kmr | Novagen |
pCESTb | estI with BamHI and NotI cloned into BamHI-NotI sites of pET29a; Kmr | This study |
pCM110 | M. extorquens expression vector; Tcr | 39 |
pCESTc | estI with SpeI and ClaI cloned into SpeI-ClaI sites of pCM110; Tcr | This study |
pPICZ B | Pichia expression vector; Zeor | Invitrogen Canada |
pCESTd | estI with KpnI and NotI cloned into KpnI-NotI sites of pPICZ B; Zeor | This study |
Ampr, ampicillin resistant; Emr, erythromycin resistant; Kmr, kanamycin resistant; Tcr, tetracycline resistant; Zeor, zeocin resistant.