TABLE 2.
Substrate | Km (μM) | 102kcat (S−1) | kcat/Km (S−1 · μM−1) |
---|---|---|---|
pNP-acetate | 134 | 3.16 | 2.35 |
pNP-butyrate | 97 | 9.88 | 10.18 |
pNP-caproate | 19 | 11.94 | 62.80 |
pNP-caprylate | 14 | 12.45 | 88.91 |
pNP-caprate | 43 | 8.96 | 20.84 |
pNP-laurate | 186 | 2.41 | 1.29 |
pNP-myristate | — | — | — |
pNP-palmitate | — | — | — |
Enzyme activity, determined at 37°C in 50 mM phosphate buffer (pH 7.0), is a mean from two or three independent experiments with less than 8% standard deviation. —, not detected.