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. 2014 Aug 28;234(3):302–315. doi: 10.1002/path.4391

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© 2014 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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OSR1 is silenced or down-regulated by promoter methylation in gastric cancer cells. (A) Expression of OSR1 mRNA in human organ tissues was determined by semi-quantitative reverse transcriptase (RT)-PCR. (B1) Expression of OSR1 mRNA in gastric cancer cell lines and normal gastric epithelial cell line. (B2) Expression of OSR1 protein in gastric cancer cell lines and normal gastric epithelial cell line. (C1) Treatment with the demethylating agent 5-aza-2'-deoxycytidine (5-Aza) restored OSR1 expression in gastric cancer cell lines. (C2) Combination treatment with 5-Aza and the histone deacetylase inhibitor trichostatin A (TSA) restored OSR1 expression in MKN45 and SNU16. (D) A typical CpG island spans the promoter region of OSR1. The transcription start site (TSS) and the regions of bisulphite genomic sequencing (BGS) and methylation-specific PCR (MSP) are indicated. Methylation status of OSR1 in the gastric cancer cell lines was determined by BGS and MSP (M, methylated; U, unmethylated).