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. 2014 Aug 28;234(3):302–315. doi: 10.1002/path.4391

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© 2014 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Knockdown of OSR1 by OSR1-shRNA-1 (A–D) and OSR1-shRNA-2 (E–G) enhances cell growth. (A1) Knockdown of OSR1 mRNA was revealed by semi-quantitative RT-PCR (shCt, shRNA control vector; shOSR1-1, OSR1-shRNA-1). (A2) Knockdown of OSR1 protein was confirmed by western blotting. (A3) Knockdown efficiency of OSR1 mRNA was examined by quantitative real-time PCR. Each column represents the mean ± SD. ^**p < 0.01. (B) Effect of OSR1 knockdown on cell viability was determined by MTS assay. Each column represents the mean ± SD. ^**p < 0.01. (C) Effect of OSR1 knockdown on cell proliferation was determined by xCELLigence analysis. Data are shown as mean ± SD. (D) Effect of OSR1 knockdown on colony numbers was determined by colony formation assay. Quantitative analysis of colony formation efficiency (%) is shown in the right panel. Each column represents the mean ± SD. ^**p < 0.01. (E1) Knockdown of OSR1 mRNA was revealed by semi-quantitative RT-PCR (shCt, shRNA control vector; shOSR1-2, OSR1-shRNA-2). (E2) Knockdown of OSR1 protein was confirmed by western blotting. (E3) Knockdown efficiency of OSR1 mRNA was examined by quantitative real-time PCR. Each column represents the mean ± SD. ^**p < 0.01. (F) Effect of OSR1 knockdown on cell viability was determined by MTS assay. Each column represents the mean ± SD. ^**p < 0.01. (G) Effect of OSR1 knockdown on colony numbers was determined by colony formation assay. Quantitative analysis of colony formation efficiency (%) is shown in the right panel. Each column represents the mean ± SD. ^**p < 0.01.