FIG. 4.
Cytosolic pH (pHcyt) (•) and vacuole pH (pHvac) (○) of A. niger NW131 in 25 mM citric-phosphate buffer (pH 4.0) at 30°C, containing 3.0 mM sorbic acid. The arrow indicates the time of addition of sorbic acid. Vertical bars show SEM (n = 2) calculated from independent cultures. Control experiments showed that the presence of ethanol in the perfusion buffer had no visible effects on pHcyt or pHvac and are therefore not presented.
