TABLE 1.

MIC of sorbic acid needed to completely inhibit spore germination or growth of young mycelia after 72 h of culture^a

Inoculum type	Inoculum size	MIC (mM)
Spore	105/ml of medium	4.5
	104/ml of medium	3.0
	103/ml of medium	2.5
	102/ml of medium	2.0
	10/ml of medium	<1.5
Mycelia	1 × 24-h culture	1.5
	2 × 24-h culture	2.0
	3 × 24-h culture	2.0

^aFor spore MIC determinations, different concentrations of conidia were inoculated in 100 ml of ACM (pH 4.0) containing sorbic acid. Mycelial inocula were propagated from 10⁵ spores/ml in 100 ml of ACM (pH 4.0) grown for 24 h. Fresh ACM (pH 4.0) containing sorbic acid was inoculated with the combined biomass from one (i.e., 1 × 24-h culture), two, or three of the initial 24-h cultures. All culturing took place in 250-ml shake flasks incubated at 28°C with shaking at 160 rpm. Results presented are from independent cultures (n = 3 for each).