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. Author manuscript; available in PMC: 2015 Jun 18.
Published in final edited form as: Neuron. 2014 Jun 18;82(6):1299–1316. doi: 10.1016/j.neuron.2014.05.016

Figure 7. Triad3A is Required for Activity-Induced Upscaling of Synaptic Strength.

Figure 7

(A) Representative images of DIV21–24 hippocampal neurons expressing GFP, GFP-Triad3A, Scr shRNA, or Triad3-shRNA treated in the presence of 1 μM TTX for 48 h. Surface AMPA receptors (sGluA1) were visualized by live GluA1 antibody labeling and subsequent fixation under non-permeabilized conditions. Scale bars, 10 μm.

(B–C) Quantitative analysis of surface GluA1 labeling on hippocampal neurons expressing GFPTriad3A (B) or Triad3-shRNA (C). Data represent means ± SEM. *p < 0.05, n = 6–23. **p < 0.005, n = 6–23.

(D) mEPSC recordings from DIV17–21 neurons expressing GFP, GFP-Triad3A or Triad3-shRNA treated with 1 μM TTX for 48 h. Note the increase in mEPSC amplitudes in GFP-expressing neurons upon TTX treatment that is prevented by Triad3A-shRNA and occluded by Triad3A overexpression.

(E) Cumulative mEPSC amplitude distribution summarizing data from GFP (top), GFP-Triad3A (middle), and Triad3-shRNA (bottom) expressing cells in control and following TTX treatment.

(F) Cumulative mEPSC inter-event interval distribution summarizing data from GFP (top), GFPTriad3A (middle), and Triad3-shRNA (bottom) expressing cells in control and following TTX treatment. Note that mEPSC frequency is not affected by GFP-Triad3A or Triad3-shRNA.

(G) Data represent means ± SEM of mEPSC amplitudes. *p < 0.05, n = 11–13.

(H) Data represent means ± SEM of mEPSC frequencies, n = 11–13.