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. 2014 Nov 17;136(48):16700–16703. doi: 10.1021/ja5073146

Figure 4.

Figure 4

Crowding agents accelerate ribozyme cleavage. (a) Fraction of intact substrate (S) and cleaved product (P) as a function of time (0–180 min) after separation by 20% denaturing polyacrylamide gel electrophoresis. The fraction of P increases with time. (b) Fraction of cleaved product as a function of time in the presence and absence of 25% PEG. The presence of PEG increases the fraction of cleaved product. (c) Amplitude of the fast and slow cleavage phases as a function of %PEG. The slow cleaving fraction decreases with increasing PEG concentration.