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Comparison of ΔΔGunf°(bR) from kinetics to ΔΔGunf°(bO) from equilibrium measurements. The effect of mutation on the free energy of unfolding as measured via the kinetic model to the bRU reference state or obtained from equilibrium methods to the bO reference state. A weighted linear fit to the data is shown (slope = 0.97, intercept = 0.26 kcal/mol, R2 = 0.88).
