Table 3. Activator Response Function Parameters.
name | inducer | Ka | n | ymaxb | yminb | fold-changec |
---|---|---|---|---|---|---|
AmtRA | Dox | 0.1 | 3.00 | 1.3 × 103 | 15 | 82 |
QacRA | DAPG | 4.6 | 1.89 | 1.1 × 104 | 122 | 91 |
The threshold at which the buffer gate is at the half-maximum output, in μM doxycyline (for AmtRA) or μM DAPG (for QacRA).
The maximum and minimum levels of expression, in arbitrary units of YFP fluorescence.
The fold-change is calculated by dividing the maximum average fluorescence (20 μM Dox or 30 μM DAPG) by the fluorescence of cells containing no inducer.