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NIRW light-inactivated gene expression module. (A) Structure of a NIRW light-inactivated synthetic module. (B) Light-inactivated β-galactosidase activity in the E. coli T7 Express crp mutant expressing a c-di-GMP-dependent Clp transcription factor. Plates contained 0.1% arabinose, 0.015 mM IPTG, and 40 μg/mL X-gal. (C) β-galactosidase activity in liquid cultures grown in the dark or light to A600, ∼1.0.
