FIG. 7.

Quantitative detection of bacterial (E. coli) 16S rRNA molecules with the sequence-dependent rRNA cleavage method in artificially mixed 16S rRNA transcripts containing E. coli 16S rRNA and M. concilii 16S rRNA. Probe EUB338 was used as the scissor probe with a formamide concentration of 20% for the hybridization-digestion buffer. Defined (actual) percentages of E. coli rRNA in the total rRNA are plotted along the x axis, whereas the measured values of the percentages obtained by the present methods are shown along the y axis. The values on the y axis were estimated from the electropherograms of digested RNA, with corrections made with a cleavage coefficient of 0.96.