Figure 2.

AhR regulates laser-induced CNV in aged mice. (A) Choroidal flat-mounts were prepared from 11–13 month-old laser-induced CNV mice (n = 20/genotype) and stained with propidium iodide (AhR^−/−, AhR knock-out; ON, optic nerve; i–iv, wt; vii–x, AhR^−/−; and isolectin GS-IB₄, v–vi, wt; xi–xii, AhR^−/−). Representative images from four eyes/genotype are shown to demonstrate individual and merged CNV lesions (dotted circles demarcate lesions and ON, i and vii) stained with propidium iodide; vi and xii, high-magnification images of individual lesions stained with isolectin GS-IB₄. (B) Distribution of number of animals with individual versus merged lesions in wt and AhR^−/− (n = 18/genotype). (C) Relative lesion area/animal was measured using ImageJ (mean and SEM; n = 18/genotype; ^**p < 0.001). (D) Relative lesion thickness/animal (mean, SEM and data points for each group; n = 9/genotype; ^***p < 0.0001); inserts display representative 3D reconstruction images for wt and AhR^−/− mice. (E) Relative lesion volume/animal (mean and SEM for each group; n = 5/genotype; ^*p < 0.05)