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. 2004 Jun;70(6):3512–3520. doi: 10.1128/AEM.70.6.3512-3520.2004

FIG. 2.

FIG. 2.

Specificity of the primer pair CIRC1A-CIRC4A for the amplification of a 360-bp fragment in the nuclear ribosomal IGS region of F. circinatum. An ethidium bromide-stained agarose gel of PCR products amplified from stains belonging to the American clade of the G. fujikuroi complex and two environmental samples from spore traps is shown. Lanes: 1, 100-bp DNA ladder (Promega); 2, water negative control; 3, F. circinatum NRRL 25331; 4, F. circinatum NRRL 25332; 5, F. circinatum NRRL 25333; 6, F. circinatum NRRL 25621; 7, F. circinatum NRRL 26431; 8, F. circinatum NRRL 26432; 9, F. circinatum NRRL 25707; 10, environmental sample from San Francisco, Calif. (site I); 11, environmental sample from San Bruno, Calif. (site II); 12, F. subglutinans NRRL 22016; 13, Fusarium sp. strain NRRL 25204; 14, Fusarium sp. strain NRRL 25622; 15, Fusarium sp. strain NRRL 26756; 16, Fusarium sp. strain NRRL 26757; 17, Fusarium sp. strain NRRL 25346; 18, Fusarium sp. strain NRRL 25623; 19, Fusarium sp. strain NRRL 25195; 20, Fusarium sp. strain NRRL 25807; 21, Fusarium sp. strain NRRL 29123; 22, F. bulbicola NRRL 13618; 23, F. bactridioides NRRL 20476; 24, F. succisae NRRL 13613; 25, F. anthophilum NRRL 13602; 26, F. begoniae NRRL 25300; 27, water negative control; 28, DNA ladder.