FIG. 6.

Quantification of F. circinatum from spore traps collected after 2 weeks in the field at San Francisco (site I) and San Bruno (site II), Calif. Three spore collection methods were used: 1, trapping with FS medium; 2, trapping with filter paper and subsequent transfer of the paper to FS medium; 3, trapping on filter paper and subsequent real-time PCR with the primer pair CIRC1A-CIRC4A. (A) Numbers of spores were calculated individually for each site, sampling point, and sampling method. (B) Cumulative numbers of spores for each sampling method combining the numbers from all sites and sampling points (vertical bars represent standard errors). Spore counts obtained by method 3 are significantly different from spore counts obtained by methods 1 and 2 (multiple-comparison analysis of variance with the Tukey-Kramer honestly significant difference test, P = 0.05).