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. Author manuscript; available in PMC: 2014 Dec 28.
Published in final edited form as: Circ Heart Fail. 2014 May 8;7(4):663–672. doi: 10.1161/CIRCHEARTFAILURE.114.001266

Figure 1.

Figure 1

Muscle A-kinase anchoring protein β (mAKAPβ) is important for pressure overload–induced hypertrophy. A, Frozen left ventricular (LV) tissue sections from MCM+tamoxifen (Tam) control (top left 2 panels) and mAKAP CKO (bottom left 2 panels) mice were stained with mAKAP FL100 antibody (gray scale panels and green), Hoechst nuclear stain (blue), and wheat germ agglutinin (red, shown in enlarged control image only). Bar, 20 μm (n=3). B–I, Mice were subjected to 2-week transverse aortic constriction (TAC) or sham operation. mAKAP CKO mice were mAKAPfl/fl;MCM+Tam. MCM+Tam and mAKAPfl/fl;MCM were controls. B and C, M-mode echocardiography for LV posterior wall thickness in diastole (LVPW;d) and ejection fraction; cf Table I in the Data Supplement. D, Biventricular weight indexed to body weight; cf Table II in the Data Supplement. E and F, Myocyte cross section area was determined by wheat germ agglutinin staining. Bar, 50 μm (n=4–6). G–I, Isolated adult myocytes were measured for width and length for the mAKAP CKO and MCM+Tam cohorts. Bar=20 μmol/L (n=3–5 mice); >100 myocytes assayed per mouse. *Vs sham-operated for same genotype; †vs MCM+Tam; ‡vs mAKAPfl/fl;MCM.