TABLE 2.
Transcriptional activity of nifH-lacZ fusions in free-living cultures and nodules
| Strain | Sp act of β-galactosidase (nmol of ONP min−1 mg of protein−1) ina: |
|||
|---|---|---|---|---|
| Free living cultures |
Nodules |
|||
| 1% O2 | 20% O2 | 11 dpi | 18 dpi | |
| CFN42 | 7 ± 2 | 9 ± 2 | NA | 65 ± 15 |
| HP310 lac (nifHc-lacZ) | 363 ± 75 | 82 ± 10 | 492 ± 137 | 929 ± 136 |
| DEM153 (nifHa-lacZ) | 136 ± 25 | 20 ± 3 | 276 ± 57 | 372 ± 190 |
| DEM233 (nifHc-lacZ) | 426 ± 40 | 21 ± 5 | 1,005 ± 143 | 1,158 ± 358 |
a
Data from free-living cultures are the averages of three different experiments. Nodules from five plants per strain per day were analyzed. Values are means ± standard deviations. NA, not analyzed; ONP, o-nitrophenol.