Table 1.
Control | PIP2 | ΔE | p | ΔE(Model)* | |
---|---|---|---|---|---|
R151C-AD | 0.855±0.002 | 0.885±0.002 | +0.030 | 0.001 | + |
R165C-AD | 0.788±0.002 | 0.816±0.003 | +0.028 | 0.001 | + |
R165C-ED | 0.054±0.029 | 0.147±0.004 | +0.093 | 0.010 | + |
A177C-AD | 0.794±0.005 | 0.880±0.002 | +0.086 | 0.001 | + |
A177C-ED | 0.286±0.010 | 0.351±0.023 | +0.065 | 0.001 | + |
A180C-AD | 0.815±0.003 | 0.835±0.005 | +0.020 | 0.001 | + |
A186C-AD | 0.795±0.003 | 0.831±0.003 | +0.036 | 0.001 | + |
K191C-AD | 0.628±0.004 | 0.711±0.002 | +0.083 | 0.001 | − |
H219C-AD | 0.463±0.004 | 0.472±0.001 | +0.009 | 0.018 | + |
G225C-ED | 0.483±0.003 | 0.508±0.005 | +0.025 | 0.043 | + |
M228C-AD | 0.796±0.008 | 0.835±0.002 | +0.039 | 0.001 | + |
S235C-AD | 0.619±0.010 | 0.763±0.008 | +0.144 | 0.004 | + |
G249C-AD | 0.451±0.002 | 0.407±0.006 | −0.043 | 0.001 | − |
G249C-ED | 0.260±0.033 | 0.213±0.011 | −0.047 | 0.099 | − |
M252C-AD | 0.668±0.003 | 0.644±0.008 | −0.024 | 0.001 | − |
E258C-AD | 0.853±0.002 | 0.868±0.003 | +0.015 | 0.001 | + |
S260C-ED | 0.616±0.014 | 0.674±0.028 | +0.058 | 0.071 | + |
T264C-AD | 0.886±0.001 | 0.898±0.006 | +0.011 | 0.083 | + |
A273C-AD | 0.350±0.005 | 0.324±0.002 | −0.026 | 0.013 | − |
A273C-ED | 0.184±0.005 | 0.153±0.001 | −0.032 | 0.001 | − |
D277C-ED | 0.321±0.022 | 0.261±0.028 | −0.061 | 0.093 | − |
R280C-ED | 0.362±0.007 | 0.329±0.005 | −0.033 | 0.002 | − |
H283C-ED | 0.111±0.006 | 0.102±0.001 | −0.009 | 0.056 | + |
T306C-ED | 0.427±0.005 | 0.485±0.009 | +0.058 | 0.001 | + |
P308C-AD | 0.837±0.010 | 0.874±0.004 | +0.037 | 0.004 | + |
Direction of FRET efficiency changes predicted by the ‘cartoon’ model of gating shown in Fig. 5, ‘+’ indicates increased FRET efficiency for closed versus open (see text). The FET efficiencies with (PIP2) or without (Control) are presented as mean ± s.e.m, n=6.