Table 1.

Changes of FRET efficiencies of labeled KirBac1.1 mutants in the presence or absence of PIP₂

	Control	PIP2	ΔE	p	ΔE(Model)*
R151C-AD	0.855±0.002	0.885±0.002	+0.030	0.001	+
R165C-AD	0.788±0.002	0.816±0.003	+0.028	0.001	+
R165C-ED	0.054±0.029	0.147±0.004	+0.093	0.010	+
A177C-AD	0.794±0.005	0.880±0.002	+0.086	0.001	+
A177C-ED	0.286±0.010	0.351±0.023	+0.065	0.001	+
A180C-AD	0.815±0.003	0.835±0.005	+0.020	0.001	+
A186C-AD	0.795±0.003	0.831±0.003	+0.036	0.001	+
K191C-AD	0.628±0.004	0.711±0.002	+0.083	0.001	−
H219C-AD	0.463±0.004	0.472±0.001	+0.009	0.018	+
G225C-ED	0.483±0.003	0.508±0.005	+0.025	0.043	+
M228C-AD	0.796±0.008	0.835±0.002	+0.039	0.001	+
S235C-AD	0.619±0.010	0.763±0.008	+0.144	0.004	+
G249C-AD	0.451±0.002	0.407±0.006	−0.043	0.001	−
G249C-ED	0.260±0.033	0.213±0.011	−0.047	0.099	−
M252C-AD	0.668±0.003	0.644±0.008	−0.024	0.001	−
E258C-AD	0.853±0.002	0.868±0.003	+0.015	0.001	+
S260C-ED	0.616±0.014	0.674±0.028	+0.058	0.071	+
T264C-AD	0.886±0.001	0.898±0.006	+0.011	0.083	+
A273C-AD	0.350±0.005	0.324±0.002	−0.026	0.013	−
A273C-ED	0.184±0.005	0.153±0.001	−0.032	0.001	−
D277C-ED	0.321±0.022	0.261±0.028	−0.061	0.093	−
R280C-ED	0.362±0.007	0.329±0.005	−0.033	0.002	−
H283C-ED	0.111±0.006	0.102±0.001	−0.009	0.056	+
T306C-ED	0.427±0.005	0.485±0.009	+0.058	0.001	+
P308C-AD	0.837±0.010	0.874±0.004	+0.037	0.004	+

^*Direction of FRET efficiency changes predicted by the ‘cartoon’ model of gating shown in Fig. 5, ‘+’ indicates increased FRET efficiency for closed versus open (see text). The FET efficiencies with (PIP₂) or without (Control) are presented as mean ± s.e.m, n=6.