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. Author manuscript; available in PMC: 2014 Dec 28.
Published in final edited form as: Biochim Biophys Acta. 2008 Sep 30;1790(1):31–39. doi: 10.1016/j.bbagen.2008.09.006

Fig. 3. Morelloflavone inhibits multiple migration-related kinases.

Fig. 3

(A) Phosphorylation of FAK and c-Src. FAK, total focal adhesion kinase; p-FAK, phosphorylated FAK; c-Src, total c-Src; p-c-Src, phosphorylated c-Src. Morelloflavone decreased phosphorylation of FAK and c-Src. The posphorylation indices of a certain kinase (such as p-FAK/FAK) were calculated by dividing the signal intensity of the band of the phosphorylated kinase by the signal intensity of the band of the total kinase, at a given morelloflavone concentration. The indices of untreated cells were normalized to 100. (B) Phosphorylation of ERK. ERK, total ERK; p-ERK, phosphorylated ERK; Morelloflavone decreased phosphorylation of ERK. (C) Activation of RhoA, Rac1, and Cdc42. Morelloflavone blocks the activation of RhoA at 0.1 μM, Cdc42 at 10 μM; but it has no significant effect on Rac1 or Cdc42 activation.