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. 2014 Aug 30;9(1):270–281. doi: 10.1016/j.molonc.2014.08.008

Figure 3.

Figure 3

D2R agonist treatment decreases angiogenesis and promotes apoptosis in the endothelium of LLC1 tumor bearing mice. A: Immunohistochemistry was performed using a monoclonal CD31 antibody on formalin‐fixed, paraffin‐embedded tissue harvested from LLC1 tumor bearing mice treated with vehicle (left) or 10 mg/kg quinpirole (right) daily for seven days. B: The amount of CD31‐positive endothelial cells in lung tissue harvested from control and quinpirole‐treated mice (n = 2 for each group) was quantitated by counting the number of CD31‐positive endothelial cells per visual field (10 visual fields were counted for each tissue). C: Co‐immunofluorescence staining for TUNEL (green; bottom left single stain) and CD31 (red; bottom right single stain) was performed on tissue harvested from LLC1 tumor bearing mice treated with vehicle (left) or 10 mg/kg quinpirole (right) daily for seven days. Merged images are shown in the top panel (green: TUNEL, red: CD31, blue: DAPI). D: Colocalization of TUNEL and CD31 staining was quantitated in the immunofluorescent images of lung tissue harvested from control and quinpirole‐treated mice (n = 2 for each group) by counting the number of double positive TUNEL and CD31 endothelial cells per visual field (10 visual fields were counted for each tissue).