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. 2014 Jul 29;9(1):44–57. doi: 10.1016/j.molonc.2014.07.013

Figure 3.

Figure 3

Co‐culture of T cells promoted PCa invasion. A. CMs collected from PCa/T cells co‐culture could promote PCa invasiveness using in vitro Matrigel invasion assay. The PCa/T cells were co‐cultured in 0.4 μM transwell plate for 2 days. The CM or control media were collected, diluted 1:1 with 10% FBS RPMI media, and placed into the lower chamber of transwells. The 1 × 105 PCa cells (without any pre‐treatment) were plated onto upper insert chamber pre‐coated with Matrigel for 36 h invasion assay. B. Toluidine blue staining results showed the PCa/T cells co‐cultured CM can promote PCa cell invasion. Invasion assay has been performed on 3 different PCa cell lines, LNCaP, C4‐2 and CWR22RV1 (22RV1) and 2 different T cells, Molt‐3 and HH (*P < 0.05). C. 3D invasion assay showed that more acini‐like structures formed when treating the parental CWR22RV1 cells with CWR22RV1/T cell co‐culture CM (200×) (*P < 0.05).