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. 2014 Jul 29;9(1):44–57. doi: 10.1016/j.molonc.2014.07.013

Figure 4.

Figure 4

T cells can down‐regulate AR expression and up‐regulate MMP9 expression of PCa cells after co‐culture. A. Q‐PCR detection showed AR mRNA was down‐regulated in PCa cells, LNCaP, C4‐2 and CWR22RV1 (22RV1), after co‐culture with HH or Molt‐3 cells for 2 days (*P < 0.05). B. Western‐blot results showed AR expression of LNCaP, C4‐2 and 22RV1 cells decreased after co‐culture with HH or Molt‐3 cells for 2 days. C. T cell co‐culture could not effectively change invasion in PCa cells with AR knockdown by siRNA. We used lentiviral AR‐siRNA to knock‐down AR expression in PCa. T cells co‐culture failed to promote invasion of PCa cell with AR‐siRNA compared with PCa cell with control si‐Luc (*P < 0.05). D. Four metastasis–related genes were detected by q‐PCR. TGF‐beta1, MMP9 and ZEB1 mRNA level were up‐regulated in PCa cells after co‐culture with T cells, however the MMP9‐inhibitor gene, TIMP1, decreased in PCa cells (*P < 0.05). E. MMP9 expressions of LNCaP, C4‐2 and 22RV1 cells are up‐regulated after co‐culture with HH or Molt‐3 cells using Western‐blot assay (*P < 0.05). F. Blocking MMP9 by MMP9 inhibitor can partly reverse T cell‐promoted PCa invasion (*P < 0.05).