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. 2014 Jul 29;9(1):44–57. doi: 10.1016/j.molonc.2014.07.013

Figure 5.

Figure 5

Down‐regulation of AR in PCa cells by T cells secreted FGF11. A. Results showed FGF11 expression is selectively and significantly increased in T cells after co‐culture with PCa cells for 2 days. The data have been validated in 2 different T cell lines co‐cultured with C4‐2 cells (*P < 0.05). B. Validation of FGF11‐siRNA knockdown efficiency in 2 different T cell lines (*P < 0.05). C. T cell promotion of PCa cell invasion can be partly reversed by FGF11 knock‐down in 2 T cell lines. The results have been validated in 2 different T cells and 3 PCa cells, LNCaP, C4‐2, and CWR22RV1 (22RV1) cells (*P < 0.05). D. Knockdown of FGF11 in T cells can partially reverse the T cells‐mediated AR down‐regulation and MMP9 up‐regulation. Results have been validated in the 2 different T cells and 3 PCa cells. E. Q‐PCR results showed that miRNA‐541 (Mir541) and miRNA‐876 (Mir876) stably reduced in PCa cells after knockdown of FGF11 in T cells co‐culture with PCa cells (*P < 0.05). F. MiRNA‐541 inhibitor can partly reverse the HH cell‐enhanced PCa cell invasion (*P < 0.05). G. Inhibition of miRNA‐541 (but not miRNA‐876) can reverse the HH cells‐mediated AR down‐regulation and MMP9 up‐regulation.