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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Exp Parasitol. 2014 Nov 13;0:17–23. doi: 10.1016/j.exppara.2014.11.004

Figure 2. Gene-specific silencing of Ov-grn-1 in Opisthorchis viverrini following delivery of dsRNA by square wave electroporation.

Figure 2

Real-time qPCR analysis revealed the transcript levels of Ov-grn-1 normalized to levels of Ov-actin in flukes after electroporation and soaking with Ov-grn-1 or luciferase (Luc) dsRNAs. Ov-grn-1 transcript levels had decreased significantly by day 1 and were not detectable by day 7. Ov-grn-1 RT-PCR products amplified from total RNA of adult flukes after electroporation with either Ov-grn-1or Luc dsRNAs (inset). Data are represented by mean ± 1 standard deviation; *** denotes statistical significance at the level of P ≤ 0.001.