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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Metabolism. 2014 Oct 25;64(2):263–273. doi: 10.1016/j.metabol.2014.10.020

Figure 4.

Figure 4

Quantitated adhesion of THP-1 cells and HUVECs. (A) Cellular fluorescence was measured by SpectraMax M5 Multi-Mode Microplate Reader with each experimental condition done in triplicate and adhesion compared to the value obtained with TNF-α only. N=7, 13, 13 for HDLControl, HDLCKD and HDLESRD, respectively. Bars show median and interquartile range values. Comparison of HDL effects among control, CKD, ESRD subjects used Kruskal-Wallis test (p value shown in upper right corner of each panel) and unpaired t-test (shown as bars between individual groups). (B) Microscopic appearance of adhesion of calcein-labeled THP-1 to TNF-α-exposed HUVEC (Green: THP-1; Blue: DAPI).