Figure 6.

HDL effects on endothelial cells survival, apoptosis, and necrosis was measured by flow cytometry using FITC Annexin V Apoptosis Detection Kit with 7-AAD showing similar effects among HDL^Control, HDL^CKD, and HDL^ESRD. HDL samples pooled in each group using the same quantity of HDL protein concentration and each experiment was done in triplicate and repeated 2–4 times. Bars show median and interquartile range values. Comparison of HDL effects among control, CKD, ESRD subjects used Kruskal-Wallis test (p value shown in upper right corner of each panel) and unpaired t-test (shown as bars between individual groups).