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Biofilm development and cell death of the P. tunicata wild-type strain. Biofilms were stained with the BacLight LIVE/DEAD bacterial viability kit. Red propidium iodide-stained cells have a compromised cell membrane and are dead. Time points after inoculation are shown as follows: (A) 1 h; (B) 24 h; (C) 48 h; (D) 72 h; (E) 144 h; (F) 168 h. Bars, 50 μm.
