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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Transpl Immunol. 2014 Nov 5;32(1):51–60. doi: 10.1016/j.trim.2014.10.005

Figure 8. Adoptive transfer of CD8+ T cells restores impaired viral clearance, restores CD8+ T cell numbers, and decreases PD-1 expression in the lung after allogeneic BMT.

Figure 8

CD8+ T cells were isolated on Day 15 post-inoculation (p.i.) from splenocytes of nontransplanted C57Bl/6 mice that were infected with 105 PFUs of SeV and adoptively transferred into AlloSeV or SynSeV on Day 8 post-inoculation. After adoptive transfer of CD8+ T cells, AlloSeV mice had significantly less virus in the lungs with levels comparable to levels in SynSeV at Day 15 p.i. A) Schematic of adoptive transfer. B) Lung tissue was analyzed by real time PCR for SeV nucleocapsid protein RNA on Day 15 p.i. C-F) Lung sections were stained for parainfluenza virus type 1 at Day 15 p.i. to further verify RNA analysis. Representative immunohistochemistry staining for SeV (green=SeV, blue=DAPI; images are 200x) C) SynSeV Day 15 p.i.; D) AlloSeV Day 15 p.i; E) SynSeV + CD8+ T cells Day 15 p.i.; F) AlloSeV + CD8+ T cells Day 15 p.i; G) CD3+CD8+ population within small cells for each experimental group on Day 15 post-inoculation. H) Percentage of CD3+CD8+ T cells that are PD-1+ for each experimental group. I) BAL fluid was analyzed by ELISA for Granzyme B levels at Day 15 post-inoculation in Allo and Syn with or without adoptive transfer of CD8+ T cells; (* = p-value less than 0.05 comparing SynSeV to AlloSeV, ns= not significant) (n=5/group, graph is representative of 1 experiment).