TABLE 3.
Expression of efflux pumps in response to different aliphatic alcoholslegend
| Efflux pump | Activity (mean ± SD) after growth on:
|
|||
|---|---|---|---|---|
| Control | Octanol | Nonanol | Decanol | |
| PttgA::lacZ | 51 ± 14 | 108 ± 13 | 114 ± 35 | 85 ± 16 |
| PttgD::lacZ | 6 ± 2 | 13 ± 6 | 18 ± 7 | 15 ± 7 |
| PttgG::lacZ | 408 ± 89 | 1,474 ± 361 | 1,585 ± 254 | 919 ± 204 |
legend
P. putida DOT-T1E bearing the pED13 (PttgA::lacZ), pMTT (PttgD::lacZ), or pANA96 (PttgG::lacZ) plasmid was grown on LB medium without or with a 1 mM concentration of the indicated aliphatic alcohol. Four hours later β-galactosidase activity (in Miller units) was determined. The results are the averages of five independent assays done in duplicate.