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. 2004 Jun;70(6):3253–3262. doi: 10.1128/AEM.70.6.3253-3262.2004

TABLE 3.

Rates of substrate utilization by TG1/pBS(Kan)ToMO expressing wild-type ToMO and TouA variants I100Q, F205G, and M180T/E284G

Enzyme Substrate utilization rates (nmol consumed/min/mg of protein)
Phenol o-Cresol m-Cresol p-Cresol Catechol
Wild type 1.8 1.1 1.0 1.4 0.75
I100Q 1.5 1.0 0.86 1.1 NDa
F205G 1.1 1.0 0.34 0.52 ND
M180T/E284G 2.8 2.2 1.5 2.2 1.1
a

ND, not determined.